Method of producing the polypeptide for treating virus-induced cancer

摘要

The present invention relates to methods of producing the polypeptides that are capable of killing cells. The polypeptides comprise a targeting agent covalently attached to a channel-forming moiety. In a preferred embodiment, the channel-forming moiety comprises a colicin and the targeting agent is a reconstructed antibody mimetic derived from monoclonal antibody variants against Epstein-Barr virus gp350/220.

主权项

1. A method of producing a fusion polypeptide for treating a cell proliferative disorder in a mammal, the fusion polypeptide comprising a cancer cell targeting agent that targets mammalian cancer cells covalently attached to a channel-forming moiety so as to have an anti-cell proliferative activity on mammalian cancer cells, wherein said channel forming moiety is a channel-forming peptide or channel-forming fragment thereof that is selected from the group consisting of an E1, Ia, Ib, A, K or N colicin, the method comprising:
culturing a host cell such that said fusion polypeptide is produced, wherein said host cell comprises a vector comprising a nucleic acid molecule encoding the fusion polypeptide.