Methods and cell cultures for promoting organogenesis and tissue development

摘要

Methods of promoting liver morphogenesis prior to the functioning of blood vessels by culturing liver cells with endothelial cells is provided. Also provided are cell cultures and method of promoting vasculogenesis of liver tissue by contacting liver cells with endothelial cells.

主权项

1. A method of promoting liver differentiation comprising
obtaining hepatic cell precursor in which no vasculature is present; directly contacting the hepatic cell precursor in vitro with endothelial cells; culturing the hepatic cell precursor and endothelial cells on a Transwell membrane in culture medium comprising Dulbecco's Modified Eagle Medium, NaHCO3, calf serum, and Matrigel thereby promoting liver differentiation and de novo vascular development.