| 摘要 |
There is provided a method of nucleic acid analysis which allows analysis of genetic diversity in multiple populations to be performed rapidly and simultaneously. The method comprises
a) isolating nucleic acid from said sample;
b) providing at least two pairs of labelled primers, wherein each said primer pair is complementary to a marker sequence in a nucleic acid of at least one member;
c) amplifying the nucleic acid;
d) digesting the labelled amplified nucleic acid with at least one restriction enzyme to produce restriction fragments, and size sorting said fragments to produce a restriction fragment length profile, and
e) analysing said restriction fragment length profile so obtained,
wherein the primer pairs provided for each marker have a different sequence to the sequence of the primer pairs for each other marker, and wherein each said primer pair is uniquely labelled relative to the other primer pair(s). |
