Method for Preparing Glucans Based on Aspergillus niger

摘要

This application concerns a method for preparing glucan from Aspergillus niger, characterised in that it comprises (i) the at least partial deacetylation of the mycelium of A. niger; (ii) acid treatment of the (partially) deacetylated mycelium, preferably following purification and/or washing, to obtain insoluble glucan and soluble chitosane, which acid treatment comprises placing the deacetylated mycelium in contact with an acidic solution; (iii) the separation of the soluble chitosan on the one hand, and the insoluble glucan on the other; (iv) alkaline treatment of the glucan comprising placing the glucan in contact with an alkaline solution to cause the glucans to flocculate; and (v) drying the flocculated glucans to obtain glucan powder.;This invention further concerns the glucans thus obtained, compositions comprising them, and their uses. The glucans of the invention may be used as immunostimulants.

主权项

1. A method for preparing glucan from Aspergillus niger comprising:
(i) at least partially deacetylating the mycelium of Aspergillus niger; (ii) placing the at least partially deacetylated mycelium in contact with an acidic solution to obtain insoluble glucan and soluble chitosan; (iii) separating the soluble chitosan on the one hand, and the insoluble glucan on the other; (iv) placing the glucan in contact with an alkaline solution to cause the glucan to flocculate; and (v) drying the flocculated glucan to obtain glucan powder.