AMPLIFICATION OF TRP1 FOR SPECIFIC DETECTION OF PHYTOPHTHORA RAMORUM

摘要

Phytophthora ramorum is currently a devastating disease for many plant species and infection presents significant economic problems, and in particular has lead to devastating effects on many specie of oak trees. The present invention provides methods and kits for selective detection of Phytophthora ramorum by amplification of indole-3-glycerol-phosphate synthase N-5′-phosphoribosyl anthranilate isomerase (trp1) in order to provide a method for detection of the presence of P. ramorum infection in a biological sample.

主权项

1. A kit for the detection of Phytophthora ramorum in a biological sample, the kit comprising:
(i) an upstream and downstream amplification primer pair for amplifying a region of the P. ramorum genome within the indole-3-glycerol-phosphate synthase N-5′-phosphoribosyl anthranilate isomerase (trp1) gene where the primer pair is 5′-1 (SEQ ID NO:3) and 3′-1 (SEQ ID NO:8), or 5′-5 (SEQ ID NO:7), and 3′-1 (SEQ ID NO:8); and, (ii) non-specific amplification reagents for isothermal amplification of the trp1 gene.