EMBODIMENTS OF A PROBE AND METHOD FOR TARGETING NUCLEIC ACIDS

摘要

Disclosed embodiments concern a probe comprising one or more pairs of monomers capable of targeting a nucleic acid target. The pair of monomers may be arranged in a manner that promotes thermoinstability of the probe complex, thus producing a probe capable of locating and/or detecting a target. The probe also may comprise one or more natural or non-natural nucleotides capable of Watson-Crick base pairing with an isosequential nucleic acid target. Particular disclosed embodiments concern a method of using the disclosed probe to target nucleic acids. In particular disclosed embodiments, the probe may be incubated with a target nucleic acid and then be detected.

主权项

1. A double stranded probe, comprising:
a pair of monomers comprising a first monomer having a formulawhere each Y independently is selected from carbon, oxygen, sulfur, a triazole, oxazole, tetrazole, isoxazole, and NRb, wherein Rb is selected from hydrogen, aliphatic, aryl, heteroaliphatic, and heteroaryl; R1 and R2 are selected from hydrogen, aliphatic, aryl, aryl aliphatic, and a heteroatom-containing moiety, or R2 is selected from a heteroatom-containing functional group; R3 is a heteroatom-containing functional group; R4 is selected from any natural or non-natural nucleobase; R5 is selected from an intercalator suitable for intercalating within a nucleic acid; “optional linker” is selected from linkers comprising alkyl linkers, amide linkers, carbamate linkers, carbonate linkers, urea linkers, and combinations thereof;
a second monomer having a formulawherein Y, R1, R2R3, R4, R5, and “optional linker” are as stated for the first monomer; V is selected from carbon, oxygen, sulfur, and NRb; and n ranges from 0 to 4; and
at least one nucleotide selected from a natural nucleotide, a non-natural nucleotide, and combinations thereof.