HUMAN RARE BLOOD GROUP MULTIPLEX PCR DETECTION METHOD AND KIT

摘要

Disclosed are a human rare blood type detection method, a kit, a rapid screening method and applications thereof. By using multiple pairs of PCR specific primers directing to the SNP loci of multiple rare blood types, the SNP loci of multiple rare blood types are simultaneously detected in the same PCR reaction system; and the multiplex PCR detection method and a Pool detection method are combined to rapidly screen the human rare blood types.

主权项

1. A human rare blood group multiplex PCR detection method, taking extracted from blood samples as a sample to be tested, using multiplex pairs of specific primers for multiplex PCR amplification, and detecting amplification results through electrophoresis; wherein the specific primers are specific primers for rare blood group antigen SNP loci on blood cell surface antigen genes; and an electrophoresis result of the multiplex PCR detection method is that positive is detectable.