&Agr;-AMYLASE, GENE OF &Agr;-AMYLASE, ENGINEERING BACTERIA CONTAINING THE GENE, AND APPLICATIONS OF ENGINEERING BACTERIA

摘要

Disclosed are an α-amylase, a gene of the α-amylase, engineering bacteria containing the gene, and applications of the engineering bacteria. The present invention provides an α-1,4-starch sugar incision enzyme gene, wherein the overall length of the gene is 1569 bp and the G+C content of the gene is 67%, 522 amino acids are encoded, a nucleotide sequence is SEQ ID NO:1, and a coded incision enzyme protein amino acid sequence is SEQ ID NO:2. The engineering bacterial strain constructed by using the gene can express an α-1,4-starch sugar incision enzyme. The enzyme preparation produced by using the gene can be used for industries such as grain processing, food processing, brewing, fermenting, textile, and medicine.