Cryopreservation of Cells and Tissue for Clinical Application

摘要

Disclosed herein are methods for cryopreserving cells and tissues under clinical conditions, allowing production of viable cell products suitable for transplantation.

主权项

1. A method of clinical processing and cryopreservation of a cell-containing sample, the method comprising:
obtaining a cell-containing tissue from an individual, optionally dissociating the tissue to form a single cell suspension; suspending the cells or tissue in a cryopreservation medium; placing the cryopreservation medium containing the cells or tissue in a cryopreservation vial; cooling the vial to 4° C. and holding the vial at 4° C. for a period of time; cooling the vial at a rate of −1° C. per minute to a temperature of −80° C.; cooling the vial at a rate of −50° C. per minute to a temperature of −120° C. to −160° C., holding the vial at −120° C. for a period of time between 0.1 and 60 minutes; and transferring the vial to the vapor phase of liquid nitrogen.