METHODS FOR THE PRODUCTION OF A COSMETIC COMPOSITION FROM HATCHING FLUID AND USES THEREOF FOR IMPROVING THE COSMETIC APPEARANCE OF SKIN

摘要

The present invention relates to cosmetic compositions obtained or obtainable from Salmonidae hatching fluid, methods of producing said compositions and their use in various cosmetic applications to the skin, particularly for reducing or preventing the cosmetic appearance or prevalence of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scaling and/or transepidermal water loss in skin of a mammalian animal.

主权项

1. A method for reducing or preventing the cosmetic appearance or prevalence of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scaling and/or transepidermal water loss in skin of a mammalian animal, comprising administering a cosmetic composition to said animal, wherein said composition is obtained or obtainable by a method comprising at least the steps of:
a) suspending Salmonidae eggs in a minimal volume of water, preferably salmon eggs; b) inducing synchronized, rapid hatching of said eggs, preferably such that hatching is complete within less than 2 hours for more than 95% of the embryos; c) filtering the hatched eggs to obtain hatching fluid; and d) filtering the hatching fluid to obtain the composition, wherein the step of filtering the hatching fluid comprises at least the steps of: (i) filtering the hatching fluid using a filter with a pore size of at least 5 μm, and collecting the filtrate; (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60 μm, and collecting the filtrate; (iii) exchanging the water in the filtrate from step (ii) with a pharmaceutically acceptable buffer; (iv) filtering the solution from step (iii) using a filter with a pore size of 0.15-0.30 μm, and collecting the filtrate; and (v) preparing said cosmetic composition from the filtrate from step (iv).