METHODS AND SYSTEMS FOR ASSESSING SAMPLE BEHAVIOR IN A FLOW CYTOMETER

摘要

Methods and systems are disclosed for generating an entrainment factor in a flow cytometry sample. The methods comprise flowing a sample with a series of particles through the flow cytometer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the flow cytometer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.

主权项

1. A method for generating an entrainment factor for a flow cytometer sample, the method comprising:
flowing the sample comprising a series of particles through the flow cytometer wherein each particle is associated with a signal; detecting a series of events with a detection system in a first time interval from a stream formed from the flow cytometer; calculating an expected frequency of events in the first time interval based on a characterizing distribution; measuring an observed frequency of events within a second time interval, wherein the second time interval is within the first time interval; and generating the entrainment factor based at least in part on a ratio of the observed frequency to the expected frequency.