METHODS OF CO-DETECTING MRNA AND SMALL NON-CODING RNA
摘要
Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non-coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single-stranded nucleic acids, and detecting the NPP, for example on a microarray.
申请公布号
EP2761030(A1)
申请公布日期
2014.08.06
申请号
EP20120834920
申请日期
2012.09.26
申请人
HTG MOLECULAR DIAGNOSTICS, INC.
发明人
SELIGMANN, BRUCE, A.;ROUNSEVILLE, MATT;MADDULA, KRISHNA;BOTROS, IHAB;COX, CHRIS