发明名称 |
RESISTANCE OF PLANTS TO BIOTIC AND ABIOTIC STRESSES BY OVEREXPRESSION OF PROTOCHLOROPHYLLIDE OXIDOREDUCTASE C AND ITS ISOFORMS |
摘要 |
<p>A process for the construction of plant transformation vector comprising: digesting a binary vector pCAMBIA 1304 with EcoRI and Sal I; subjecting the digested binary vector to the step of end filling by Klenow; re-ligating the said binary vector by known method; obtaining CaMV 35 8 promoter cassette with Omega enhancer from pSH9; incorporating the said Camv35S promoter cassette into the Hind III site of the said binary vector to obtain modified pCAMBIA 1304 vector; subjecting pore DNA fragment (1206 bp) to the step of amplification using a pair of primers; introducing EcoRI restriction sites to both the primers; ligating the said amplified cDNA fragment to pGEM T-easy; extracting EcoRI digested porC cDNA fragment from cloned pGEM T-easy; inserting said EcoRI digested porC cDNA fragment into modified pCAMBIA 1304 plant transformation vector to produce recombinant plasmids construct (pCAMBIA 1304-AtporC).</p> |
申请公布号 |
EP2052080(B1) |
申请公布日期 |
2014.08.06 |
申请号 |
EP20070790098 |
申请日期 |
2007.07.24 |
申请人 |
DEPARTMENT OF BIOTECHNOLOGY;JAWAHAR LAL NEHRU UNIVERSITY |
发明人 |
TRIPATHY, BAISHNAB CHARAN |
分类号 |
C12N15/29;C12N9/02;C12N15/53;C12N15/82 |
主分类号 |
C12N15/29 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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