发明名称 |
Preparation of maytansinoid antibody conjugates by a one-step process |
摘要 |
The invention provides a one-step process for preparing a cell-binding agent cytotoxic agent conjugate comprising contacting a cell-binding agent with a cytotoxic agent to form a first mixture comprising the cell-binding agent and the cytotoxic agent and contacting the first mixture comprising the cell-binding agent and the cytotoxic agent with a bifunctional crosslinking reagent, which provides a linker, in a solution having a pH of about 4 to about 9 to provide a second mixture comprising the cell-binding agent cytotoxic agent conjugate, wherein the cell-binding agent is chemically coupled through the linker to the cytotoxic agent, free cytotoxic agent, and reaction by-products. The second mixture is then optionally subjected to purification to provide a purified cell-binding agent cytotoxic agent conjugate. |
申请公布号 |
US8795673(B2) |
申请公布日期 |
2014.08.05 |
申请号 |
US201213434451 |
申请日期 |
2012.03.29 |
申请人 |
ImmunoGen, Inc. |
发明人 |
Li Xinfang;Worful Jared M. |
分类号 |
A61K47/48;A61K39/395 |
主分类号 |
A61K47/48 |
代理机构 |
Leydig, Voit & Mayer, Ltd. |
代理人 |
Leydig, Voit & Mayer, Ltd. |
主权项 |
1. A process for preparing an antibody maytansinoid conjugate comprising the step of:
(a) contacting an antibody with a maytansinoid to form a first mixture comprising the antibody and the maytansinoid, then contacting the first mixture with a bifunctional crosslinking reagent comprising a linker, in a solution having a pH of about 4 to about 9 to provide a second mixture comprising (i) the antibody maytansinoid conjugate, wherein the antibody is chemically coupled through the linker to the maytansinoid, (ii) free maytansinoid, and (iii) reaction by-products. |
地址 |
Waltham MA US |