发明名称 |
Modular functional peptides for the intracellular delivery of nanoparticles |
摘要 |
Described are nucleic acids encoding a polypeptide for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain, (b) a spacer domain, (c) an uptake domain, and (d) a vesicle escape domain, wherein the domains (a) through (d) appear in the same order as listed above, and wherein the peptide, upon addition of a non-hydrolyzable lipophilic moiety to the vesicle escape domain and binding to a nanoparticle, is effective to induce uptake of a nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots. |
申请公布号 |
US8796435(B2) |
申请公布日期 |
2014.08.05 |
申请号 |
US201313774493 |
申请日期 |
2013.02.22 |
申请人 |
The United States of America, as represented by the Secretary of the Navy;The University of Chicago |
发明人 |
Delehanty James B.;Medintz Igor L.;Mattoussi Hedi M.;Deschamps Jeffrey R.;Dawson Glyn;Dawson Philip E.;Blanco-Canosa Juan Bautista;Boeneman Kelly |
分类号 |
C07H21/02;C07H21/04;B82Y5/00;A61K47/48;A61K49/00;C07H19/00 |
主分类号 |
C07H21/02 |
代理机构 |
US Naval Research Laboratory |
代理人 |
US Naval Research Laboratory ;Roberts Roy |
主权项 |
1. A nucleic acid encoding a peptide selected from the group consisting of SEQ ID Nos: 3 through 6 and SEQ ID Nos: 9 through 36, inclusive. |
地址 |
Washington DC US |