Blastomere culture to produce mammalian embryonic stem cells

摘要

The present invention relates generally to the field of somatic cell nuclear transfer (SCNT) and to the creation of cloned animals and cells. The disclosure relates to a method of cloning a mammal, obtaining pluripotent cells such as embryonic stem cells, or for reprogramming a mammalian cell using an oocyte and a fertilized embryo.

主权项

1. A method of producing an mammalian embryonic stem (ES) cell, comprising
(a) culturing a mammalian blastomere for 12 to 18 hours; (b) transferring the blastomere to blastocyst medium further comprising laminin and seeded with fibroblasts; and (c) culturing the blastomere of (b) until ES cells are produced.