Recombinant enzyme with altered feedback sensitivity

摘要

The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.

主权项

1. A method for the preparation of methionine, its precursors or products derived thereof, in a fermentative process with a microorganism where L-homoserine is converted into O-succinylhomoserine with a homoserine transsuccinylase comprising the step of culturing the microorganism on an appropriate culture medium and recovering methionine, its precursors or products derived thereof once produced, wherein the homoserine transsuccinylase is a mutated homoserine transsuccinylase with reduced sensitivity for the feedback inhibitors S-adenosylmethionine and methionine, wherein the homoserine transsuccinylase has the sequence as shown in SEQ ID NO:1 and wherein the conserved amino acid Q at position 64 is replaced with a glutamic acid (E) or an aspartic acid (D).