Method for orienting tissue samples on a microtome sectionable biopsy support

摘要

Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include elongated legs coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes and orientation devices to hold and orient tissue samples for processing, embedding and microtome sectioning.

主权项

1. A method for preparing one or more elongated biopsy tissue samples for histological examination, comprising:
positioning an elongated tissue sample in a first elongated channel of a perforated microtome sectionable support, the microtome sectionable support including a tissue cassette having a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space; extending the elongated tissue sample from the first elongated channel to a second elongated channel segregated from the first elongated channel by separating structure; using biasing structure that extends into the first and second channels to hold the tissue sample against the bottom wall; subjecting the microtome sectionable support and the tissue sample to a process that replaces fluid in the tissue sample with a hardenable material; embedding the microtome sectionable support and the tissue sample in an embedding material; hardening the embedding material into a block; and slicing the block with a microtome into thin slices of the embedding material, the microtome sectionable support and the tissue sample.