Detection assay devices and methods of making and using the same

摘要

An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.

主权项

1. A biomolecular detector, comprising:
(a) a substrate having a surface portion; (b) a linking layer on said surface portion; (c) a polymer layer formed on said linking layer, said polymer comprising monomeric units, with each of said monomeric units comprising a monomer core group having at least one protein-resistant head group coupled thereto, to thereby form a brush molecule on said surface portion; said brush molecule comprising a stem formed from the polymerization of said monomer core groups, and a plurality of branches formed from said head group projecting from said stem; and (d) a first member of a specific binding pair directly non-covalently bound to said polymer layer; wherein said first member is bonded to said polymer layer at a density of from 1 milligram per meter2 to 50 grams per meter2.