发明名称 |
FREMGANGSMÅDE TIL OPRENSNING AF PEGYLERET ERYTHROPOIETIN |
摘要 |
Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl™ S 500 HR chromatography material conditioned to a conductivity of 21 mS/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield. |
申请公布号 |
DK2616101(T3) |
申请公布日期 |
2014.08.04 |
申请号 |
DK20110754693T |
申请日期 |
2011.09.13 |
申请人 |
F.HOFFMANN-LA ROCHE AG |
发明人 |
KOEHNLEIN, WOLFGANG;KUHNE, WOLFGANG;FALKENSTEIN, ROBERTO;SCHURIG, HARTMUT |
分类号 |
A61K47/48;B01D15/36 |
主分类号 |
A61K47/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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