CROSS-OVER DUAL VARIABLE DOMAIN IMMUNOGLOBULIN CONSTRUCTS

摘要

Engineered cross-over DVD-Ig binding proteins that bind to two or more target proteins (e.g., antigens) are provided, along with methods of making and uses in the prevention, diagnosis, and/or treatment of disease.

主权项

1. A binding protein comprising first and second polypeptide chains,
wherein said first polypeptide chain comprises VL1-(X1)n-VL2-CL-(X2)n, wherein VL1 is a first light chain variable domain, VL2 is a second light chain variable domain, CL is a light chain constant domain, X1 is a linker with the proviso that it is not a constant domain, and X2 is an Fc region if X4 is not an Fc region; wherein said second polypeptide chain comprises VH2-(X3)n-VH1-CH-(X4)n, wherein VH1 is a first heavy chain variable domain, VH2 is a second heavy chain variable domain, CH is a heavy chain constant domain, X1 is a linker with the proviso that it is not a constant domain, and X4 is an Fc region if X2 is not an Fc region; wherein n is 0 or 1, wherein the VL1 and VH1 domains form one functional binding site for antigen A, and wherein the VL2 and VH2 domains form one functional binding site for antigen B.