<p>A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures to optimize the expression is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of the multi-replicon single vector system for producing Rituximab in plant cells.</p>
申请公布号
WO2014116721(A1)
申请公布日期
2014.07.31
申请号
WO2014US12575
申请日期
2014.01.22
申请人
THE ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OFARIZONA STATE UNIVERSITY;MASON, HUGH;ARNTZEN, CHARLES;ROSENTHAL, SUN HEE;WINKLE, SEAN;DIAMOS, ANDY
发明人
MASON, HUGH;ARNTZEN, CHARLES;ROSENTHAL, SUN HEE;WINKLE, SEAN;DIAMOS, ANDY