RECOMBINANT HUMAN PARAOXONASE 1 ENZYMES, METHOD OF GENERATION AND USES THEREOF

摘要

The present invention provides novel polynucleotide sequences for high level expression in Escherichia coli and encoding recombinant human paraoxonase 1 enzymes having enhanced hydrolytic activity towards at least one PONl -substrates. The invention also provides a novel method to produce the recombinant enzymes in highly pure and active form in an unprecedented high yield. The invention also provides novel compositions for long-term storage stability of purified enzymes under various storage conditions. The molecules disclosed in the present invention can be used as therapeutic protein against PONl -associated disease conditions as well as agent for decontamination of OP-contaminated areas and objects.