QUANTIFICATION METHOD FOR EXPRESSION LEVEL OF WT1 mRNA

摘要

Provided is a method for quantifying, with ease, in a short time period, and with high sensitivity, human WT1 mRNA expression level which can be used to diagnose cancers such as leukaemia and solid carcinoma, and which can be used to determine bone-marrow transplantation times. This quantification method for human WT1 mRNA expression level uses one-step RT-PCR to quantify human WT1 mRNA expression level, and is characterized in that a reverse transcription reaction and an elongation reaction of human WT1 mRNA and a housekeeping gene (mRNA) are simultaneously and continuously progressed in the same vessel.