| 摘要 |
PROBLEM TO BE SOLVED: To disclose a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, in which the serum-free medium contains an inhibitor against the protease released from cultured cells.SOLUTION: In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, in which the inhibitor can be a dextran sulfate. This invention also provides a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography. |
