发明名称 |
Novel Artificial Antigen Presenting Cells and Uses Thereof |
摘要 |
The invention relates to novel artificial antigen presenting cells (aAPCs). The aAPC comprises at least one stimulatory ligand and at least one co-stimulatory ligand where the ligands each specifically bind with a cognate molecule on a T cell of interest, thereby mediating expansion of the T cell. The aAPC of the invention can further comprise additional molecules useful for expanding a T cell of interest. The aAPC of the invention can be used as an “off the shelf” APC that can be readily designed to expand a T cell of interest. Also, the aAPC of the invention can be used identify the stimulatory, co-stimulatory, and any other factors that mediate growth and expansion of a T cell of interest. Thus, the present invention provides powerful tools for development of novel therapeutics where activation and expansion of a T cell can provide a benefit. |
申请公布号 |
US2014212446(A1) |
申请公布日期 |
2014.07.31 |
申请号 |
US201414247298 |
申请日期 |
2014.04.08 |
申请人 |
The Trustees of The University of Pennsylvania |
发明人 |
Riley James L.;June Carl H.;Vonderheide Robert H.;Aqui Nicole;Suhoski Megan M. |
分类号 |
A61K39/39;A61K47/48;G01N33/569;C12N15/86;G01N33/50 |
主分类号 |
A61K39/39 |
代理机构 |
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代理人 |
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主权项 |
1. An isolated artificial antigen presenting cell (aAPC), said aAPC comprising a K562 cell transduced using a lentiviral vector (LV), wherein said LV comprises a nucleic acid encoding at least one immune stimulatory ligand and at least one co-stimulatory ligand and further wherein said aAPC expresses said stimulatory ligand and said co-stimulatory ligand and can stimulate and expand a T cell contacted with said aAPC. |
地址 |
Philadelphia PA US |