Monolayer Stress Microscopy

摘要

Disclosed are systems, apparatus, devices and methods, including a method that includes determining traction forces exerted by a cellular monolayer on a substrate on which the monolayer is placed, and determining internal forces within and between cells of the monolayer based on the determined traction forces. In some embodiments, determining the internal forces of the cellular monolayer may include determining internal stresses within the cellular monolayer that act to balance the determined traction forces over at least part of the cellular monolayer. In some embodiments, determining of the internal stresses may also include setting boundary conditions at a boundary determined based on an optical field of view of an observed section of the monolayer.

主权项

1. A method comprising:
determining traction forces exerted by a cellular monolayer on a substrate on which the monolayer is placed; and determining internal forces within and between cells of the monolayer based on the determined traction forces.