| 主权项 |
1. A method for producing a cellular systems biology profile of one or more tissue samples comprising:
a) obtaining at least two sections from one or more tissue samples; b) labeling at least one section with a histological stain, thereby producing a histologically stained section; c) labeling at least one section with a panel of fluorescently labeled reagents, thereby producing a fluorescently labeled section, wherein each fluorescently labeled reagent is specific for a biomarker, and wherein the panel of fluorescently labeled reagents detects at least four different biomarkers, and wherein the detection of a biomarker is a read-out of one or more features of a cellular systems biology profile; d) imaging the histologically stained section with at least a first optical mode, wherein the imaging produces a first set of data; e) imaging the fluorescently labeled section with at least a second optical mode, wherein the imaging produces a second set of data; f) analyzing the first set of data and the second set of data to identify five or more features, wherein at least one feature is identified in each of the first set of data and the second set of data, and wherein the combination of the five or more features generates a cellular systems biology profile of the one or more tissue samples, wherein at least the steps of e) and f) are automated, thereby providing a method for producing a cellular systems biology profile of the one or more tissue samples. |
