发明名称 |
Mast Cell Culture and Methods to Produce the Culture |
摘要 |
The present invention relates to mast cell cultures that are derived from hematopoietic progenitors and the use thereof. The invention describes a method for generating in-vitro cultures of human mast cells with functional phenotype of connective tissue-type mast cells. By monitoring the levels of chemokines released into the medium, such mast cell cultures can be used as a cell-based assay to assess regulation of mast cell functions and pharmacological activities of tryptase inhibitors. |
申请公布号 |
US2014212971(A1) |
申请公布日期 |
2014.07.31 |
申请号 |
US201414243900 |
申请日期 |
2014.04.03 |
申请人 |
Apollonian Biosystems Limited |
发明人 |
TAM See-Ying;TAM Yee San Issan;LAU Hang Yung Alaster |
分类号 |
C12N5/0787 |
主分类号 |
C12N5/0787 |
代理机构 |
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代理人 |
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主权项 |
1. A method to make a mast cell culture comprising the steps of (a) treating CD34+ progenitor cells under hypoxic condition for 1 week in a medium comprising SCF, IL-6 and IL-3 to generate an initial-stage cell culture; (b) treating the initial-stage cell culture under hypoxic condition for about 0.5-2.5 weeks in a medium comprising SCF, IL-6 and IL-9 to form an intermediate stage cell culture; (c) treating the intermediate-stage cell culture under normoxic condition for 2.5-3.5 weeks in a medium comprising SCF and IL-6 to generate an immature mast cell culture. |
地址 |
Palo Alto CA US |