MUTANT GAMMA-GLUTAMYLTRANSFERASE, AND A METHOD FOR PRODUCING GAMMA-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF

摘要

A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

主权项

1. A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein:
the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has the amino acid sequence of the positions 391 to 580 of SEQ ID NO: 2 or the amino acid sequence having a homology of 90% or more to the foregoing amino acid sequence, and has a mutation for one or more residues corresponding to one or more residues selected from the following residues in the amino acid sequence of SEQ ID NO: 2:N411, T413, Q430, P441, V443, Y444, L446, A453, D472, G484, S498, Q542, D561, S572.