发明名称 |
VERSATILE NATIVE CHEMICAL LIGATION TECHNOLOGIES |
摘要 |
Novel methods of native chemical ligation are provided. The methods involve reacting a thioacid (e.g. a peptide thioacid) with an aziridinyl compound (e.g. an aziridinyl peptide) or glycosylamine under mild conditions without the use of protecting groups, and without requiring that a cysteine residue be present in the ligation product. Initial coupling of the thioacid and the aziridinyl compound yields a ligation product containing an aziridinyl ring. Optional subsequent opening of the aziridinyl ring (e.g. via a nucleophilic attack) produces a linearized and modified ligation product. Coupling of a peptide thioacid and glycosylamine yields a glycosylated peptide. |
申请公布号 |
US2014213758(A1) |
申请公布日期 |
2014.07.31 |
申请号 |
US201414211176 |
申请日期 |
2014.03.14 |
申请人 |
Washington State University |
发明人 |
Garner Philip |
分类号 |
C07K1/107;C07H13/00 |
主分类号 |
C07K1/107 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method of forming a glycosylated ligation product, comprising
reacting a thioacid with a compound that comprises a glycosylamine under conditions suitable for forming said ligation product. |
地址 |
Pullman WA US |