发明名称 |
Expression of Honeysuckle yellow vein virus(HYVV)-C4 gene in Escherichia coli and production of polyclonal antibody against the recombinant TYLCV-C4 protein |
摘要 |
PURPOSE: A method for producing C4 recombinant protein of Honeysuckle HYVV(yellow vein virus) and a method for preparing an antibody for the recombinant protein are provided to develop a virus diagnosis kit. CONSTITUTION: A method for preparing C4 recombinant protein HYVV-C4 recombinant protein comprises: a step of amplifying HYVV-C4 gene by PCR and cloning the gene into pET28a vector; a step of transforming the vector into E.coli; a step of selecting E. coli colony and culturing; and a step of adding IPTG to express the recombinant protein; and a step of isolating and purifying the recombinant protein. |
申请公布号 |
KR101423256(B1) |
申请公布日期 |
2014.07.30 |
申请号 |
KR20110120636 |
申请日期 |
2011.11.18 |
申请人 |
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发明人 |
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分类号 |
C12N15/33;C12N15/70;C12P21/00;C12Q1/68 |
主分类号 |
C12N15/33 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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