Plant viral expression vectors and use of same for generating genotypic variations in plant genomes

摘要

A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, thereby generating genotypic variation in the genome of the plant.

主权项

1. A method of generating genotypic variation in a genome of a plant, the method comprising introducing into the plant at least one Tobacco Rattle Virus (TRV) expression vector encoding at least one chimeric nuclease which comprises a zinc finger binding domain, a nuclease, and a localization signal to a DNA-containing organelle, wherein said zinc finger binding domain mediates specific targeting of said nuclease to the genome of the plant, and wherein a nucleic acid sequence of said TRV expression vector is devoid of the 2b sequence as set forth in SEQ ID NO: 43, thereby generating genotypic variation in the genome of the plant.