Double-stranded and single-stranded RNA molecules with 5′ triphosphates and their use for inducing interferon

摘要

Double-stranded and single-stranded RNA molecules, and their use in methods for inducing interferon are provided. The interferon induction provides anti-viral and other medically useful effects, such as anti-cancer effects. Also provided are methods for reducing or inhibiting interferon induction exhibited by such molecules, particularly siRNA and shRNA molecules produced in vitro.

主权项

1. A method of inducing an anti-viral response in a cell, comprising introducing into a cell an effective amount of a siRNA molecule, wherein the siRNA molecule is double stranded, wherein the siRNA molecule has a 5′-triphosphate on each strand, wherein the siRNA molecule is targeted to a viral gene, wherein the siRNA molecule induces interferon which mediates an anti-viral response and wherein the siRNA molecule reduces expression of the targeted viral gene.