In vitro point-of-care sensor and method of use

摘要

An in vitro sensor for point-of-care detection of at least one analyte or reaction product includes an inert, impermeable substrate, a sensing system, and a reference system. The substrate includes a first transparent surface oppositely disposed from a second surface and first and second cavities. Each of the first and second cavities defines an opening at the second surface. The sensing system is disposed in at least a portion of the first cavity and includes an analyte-detection optode membrane, an analyte-permeable membrane, and a plurality of non-transparent microbeads associated with at least one of the analyte-detection optode membrane and the analyte-permeable membrane. The analyte-permeable membrane is layered upon the analyte-detection optode membrane and covers the opening of the first cavity. The reference system is disposed in at least a portion of the second cavity.

主权项

1. An in vitro sensor for point-of-care (POC) detection of at least one analyte or reaction product, said sensor comprising:
an inert, impermeable substrate including a first transparent surface oppositely disposed from a second surface and first and second cavities, each of said first and second cavities defining an opening at said second surface; a sensing system disposed in at least a portion of said first cavity, said sensing system comprising an analyte-detection optode membrane, an analyte-permeable membrane, and a plurality of non-transparent microbeads associated with at least one of said analyte-detection optode membrane and said analyte-permeable membrane, said analyte-permeable membrane being layered upon said analyte-detection optode membrane and covering said opening of said first cavity; and a reference system disposed in at least a portion of said second cavity.