METHOD FOR MAKING DERMAL MATRIX

摘要

FIELD: medicine.SUBSTANCE: method involves skin sampling from a dead donor in an operating room with using a skin grafting knife according to the standard procedure in compliance with aseptic and antiseptic regulations. Immediately after preparation, the biological material is placed in a sterile container with an aqueous solution of broad-spectrum antibiotic. The container is hermetically sealed keeping them sterile. The biomaterial is stored at -40°C until the donor's pathoanatomical findings have been obtained, and biological safety of the donor's tissues has been studied. The biomaterial with the proved biological safety is used for preparing the DM. Preparing the DM involves the following stages: separating the epidermis and the derma, conducting decellularisation of the derma, providing biocompatibility of the graft.EFFECT: method enables reducing the production process to 36 hours and provides preparing the cell-free, biocompatible dermal matrix up to 1 mm thick with preserving fibre structures and direction.2 cl, 2 tbl, 1 dwg