| 摘要 |
Disclosed is a method for the purification of a divalent cation binding protein comprising the steps of: a) loading an anion exchange resin material with the divalent cation binding protein in a loading buffer in the absence of divalent cations, and optionally washing the loaded anion exchange resin material with a washing buffer in the absence of divalent cations; and b) eluting the divalent cation binding protein with an eluant comprising at least one divalent cation to form an eluate containing the divalent cation binding protein; wherein the eluant in step (b) has a pH higher than the pH of the washing buffer in step (a), or, in case no washing step is carried out, of the loading buffer in step (a), wherein the pH of the eluant in step (b) is at least 0.5 pH units higher than the pH of the washing buffer in step (a), or, in case no washing step is carried out, of the loading buffer in step (a). |
