| 摘要 |
<p>Disclosed is a method for quantifying nucleic acids comprising: a) labelling a reference oligonucleotide having a predetermined length with a detectable marker; b) generating a standard curve using serial dilutions of the labelled reference oligonucleotide by plotting the intensity of the detectable marker against concentration of labelled reference oligonucleotide; c) amplifying a target nucleic acid in the presence of the detectable marker which labels the amplified target nucleic acid; and d) comparing in real time the intensity of the detectable marker associated with the labelled amplified target nucleic acid, with the standard curve and determining the quantity of the amplified target nucleic acid, wherein said reference oligonucleotide is not amplified or co-amplified with the target nucleic acid.</p> |
