发明名称 |
METHODS OF SYNTHESIZING HETEROMULTIMERIC POLYPEPTIDES IN YEAST USING A HAPLOID MATING STRATEGY |
摘要 |
Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein. |
申请公布号 |
US2014206039(A1) |
申请公布日期 |
2014.07.24 |
申请号 |
US201414165896 |
申请日期 |
2014.01.28 |
申请人 |
ALDER BIOPHARMACEUTICALS, INC. ;KECK GRADUATE INSTITUTE |
发明人 |
CREGG James M.;Latham John;Litton Mark;Schatzman Randal;Tolstrorukov Ilya |
分类号 |
C07K16/28 |
主分类号 |
C07K16/28 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
BOTHELL WA US |