| 摘要 |
A method and an apparatus for laser differential confocal spectrum microscopy integrate differential confocal detection and spectral detection technologies, and utilize a dichroic beam splitting system to separate Rayleigh scattering light and Raman scattering light, wherein the Raman scattering light is used in spectrum detection and the Rayleigh scattering light is used in geometric position detection. Based on a characteristic that a zero-crossing point of a differential confocal curve accurately corresponds to a focal point position, spectrum information of the focal point position of an excitation light spot is accurately captured through zero-crossing point triggering, realizing high-spatial-resolution spectrum detection, and forming the method and the apparatus that can realize high-spatial-resolution spectrum detection on a sample micro-area. The method and the apparatus have advantages of being accurate in positioning, high in spatial resolution, high in sensitivity of the spectrum detection, controllable in measuring a focal spot size and the like, and have application prospects in fields such as biological medicine, forensic evidence collection and the like. |
