SPLIT PEROXIDASES AND METHODS OF USE

摘要

An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.

主权项

1. An imaging method, comprising:
providing a sample containing a cell that expresses a split peroxidase comprising two or more separate components of a peroxidase, and contacting the sample with a peroxidase substrate to allow conversion of the peroxidase substrate into a product via an enzymatic reaction catalyzed by a reconstituted peroxidase that forms when the two or more components of the split peroxidase interact, wherein the product releases a detectable signal