CHEMILUMINESCENCE COMPACT IMAGING SCANNER

摘要

Systems, devices, and methods for accurately imaging chemiluminescence and other luminescence are disclosed. A compact, flat-bed scanner having a light-tight enclosure, one or more detector bars of linear charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS) imaging chips, and high working numerical aperture (NA) optics scans closely over a sample in one direction and then the opposite direction. Averages or other combinations of intensity readings for each pixel location (x, y) between the two or more passes are averaged together in order to compensate for luminescence that varies over time. On-chip pixel binning and multiple clock frequencies can be used to maximize the signal to noise ratio in a CCD-based scanner.

主权项

1. A method of scanning a luminescent sample, the method comprising:
providing a detector bar having one or more image sensor arrays, each image sensor array having a plurality of pixel elements; moving the detector bar with respect to a sample bed in a first pass; taking a first set of pixel readings over predetermined exposure times during the first pass, each pixel reading of the first set occurring at a respective x, y position; moving the detector bar with respect to the sample bed in a second pass, the second pass being in an opposite direction from the first pass; collecting a second set of pixel readings over predetermined exposure times during the second pass; and summing respective position x, y pixel readings between the first and second sets of pixel readings over the respective predetermined exposure times to construct an image.