Rapid Method for Detection of Pathogen

摘要

The present specification discloses methods of detecting a pathogen of interest and components useful in carrying out these methods, including a pre-enrichment media, and enrichment media and a detection solution.

主权项

1. A method of detecting a pathogen in a sample the method comprising the steps:
a) incubation of the sample in a pre-enrichment comprising a low growth nutrient component, a growth inhibiting agent, and a surfactant with bacteriostatic or bactericidal action, wherein the incubation at 37° C. for about 6 hours to about 24 hours; b) incubating an aliquot of pre-enrichment media from step (a) in an enrichment media, the enrichment media comprising a high growth nutrient component and ferric ammonium citrate, wherein the incubation at about 15° C. to about 50° C. for about 2 hours to about 24 hours; and c) detecting the presence of absence of a pathogen by analyzing an aliquot of enrichment media from step (b).