AUTOMATED SIZE SELECTION OF NUCLEIC ACIDS

摘要

Apparatus and methods for size selecting nucleic acid molecules having wide range of applications including the production of DNA libraries for sequencing technologies. An automated high throughput system for size selection of multiple nucleic acid samples that uses imaging technique to detect the progress of a target fraction and feedback from the imaging to control electrophoresis. Predictive algorithms for timed nucleic acid extractions are generated to provide size selected nucleic acid molecules of required size ranges.

主权项

1. A method for size-selection of nucleic acids, the method comprising:
moving nucleic acids from a sample along a channel by electrophoresis; automatically monitoring progress of a reference fraction of the nucleic acids along the channel; based on the monitoring, estimating an estimated time of arrival of a target fraction of the nucleic acids at an extraction well in the channel; and extracting fluid containing the target fraction from the extraction well at the estimated time of arrival.