FLUIDIC CONNECTORS AND MICROFLUIDIC SYSTEMS

摘要

Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.

主权项

1. A method of storing reagents comprising:
disposing a first reagent in a first microfluidic channel of a microfluidic system, wherein the first microfluidic channel comprises at least one inlet and one outlet, and wherein the microfluidic system comprises a second microfluidic channel including at least one inlet and one outlet; disposing a second reagent in the second microfluidic channel; and sealing the at least one inlet and outlet of the first microfluidic channel so as to store the first reagent in the first microfluidic channel, wherein the first and second microfluidic channels are not in fluid communication with one another, and wherein the first and second microfluidic channels are constructed and arranged to be in fluid communication with one another by a fluidic connector that can connect to an inlet or an outlet of the first microfluidic and to an inlet or an outlet of the second microfluidic channel.