主权项 |
1. A method of generating a spectrum of a cell, the method comprising:
(a) receiving a plurality of spectral pixels, each of the spectral pixels corresponding to a portion of the cell, each of the spectral pixels being associated with a plurality of measurements, each of the measurements being associated with an intensity of light at a particular wavenumber, one of the measurements associated with each spectral pixel being a sorting measurement, the sorting measurement being associated with a wavenumber within a band of wavenumbers; (b) identifying a subset of the plurality of the spectral pixels, a first pixel being in the subset, the sorting measurement of the first pixel being greater than or equal to the sorting measurements of the other spectral pixels, other pixels in the plurality of spectral pixels being in the subset if they satisfy a first criteria, a spectral pixel satisfying the first criteria if that spectral pixel's sorting measurement is greater than a first threshold; (c) generating the spectrum, the spectrum having a plurality of reconstructed measurements, each of the reconstructed measurements corresponding to a particular wavenumber, each of the reconstructed measurements being formed according to a sum of the measurements associated with a particular wavenumber of all the pixels in the subset. |