3D IMAGING OF LIVE CELLS WITH ULTRAVIOLET RADIATION

摘要

PROBLEM TO BE SOLVED: To provide a 3D imaging method for live cells with reduced influence on a biological object.SOLUTION: A method for 3D imaging of a biological object (1) in an optical tomography system (11) comprises: moving the biological object (1) relative to a microscope objective (18) to present varying angles of view; illuminating the biological object (1) with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm; sensing radiation transmitted through the biological object (1) and the microscope objective (18) with an ultraviolet camera (48); forming a plurality of pseudoprojections of the biological object (1) from the sensed radiation; and reconstructing the plurality of pseudoprojections to form a 3D image.