发明名称 STABLE NAD/NADH DERIVATIVES
摘要 The present invention provides for stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.
申请公布号 US2014206024(A1) 申请公布日期 2014.07.24
申请号 US201414222645 申请日期 2014.03.23
申请人 Roche Diagnostics Operations, Inc. 发明人 Heindl Dieter;Hoenes Joachim;Horn Carina;Gaessler-Dietsche Claudia
分类号 C12Q1/32 主分类号 C12Q1/32
代理机构 代理人
主权项 1. A test element configured for determining an analyte, comprising (i) a coenzyme-dependent dehydroogenase or a substrate for such an enzyme and (ii) a compound of the following general formula (I) as the coenzyme: in which A=adenine or an analogue thereof, T=in each case independently denotes O, S, U=in each case independently denotes OH, SH, BH3−, BCNH2−, V=in each case independently denotes OH or a phosphate group, W=COOR, CON(R)2, COR, CSN(R)2 in which R in each case independently denotes H or C1-C2-alkyl, X1, X2=in each case independently denote O, CH2, CHCH3, C(CH3)2, NH, NCH3, Y=NH, S, O, CH2, Z=a residue comprising a cyclic group with 5 C atoms which optionally contains a heteroatom selected from O, S and N and optionally one or more substituents, and a residue CR42 wherein CR42 is bound to the cyclic group and to X2,where R4=in each case independently denotes H, F, Cl, CH3,provided that Z and the pyridine residue are not linked by a glycosidic bond,or a salt or optionally a reduced form thereof.
地址 Indianapolis IN US