Immunochromatography Detection Sensor Comprising Optical Waveguide and a Detection Method Using the Same

摘要

The present invention relates to an immunochromatographic detection sensor comprising optical waveguides and a detection method using the same, and more particularly, to an immunochromatographic detection sensor comprising optical waveguides, in which the optical waveguides are provided under the membrane, probe beams transmitted through the optical waveguide maximize the interaction frequency between evanescent wave generated on the surface of the optical waveguide and the colored conjugate in the band formed on the membrane, resulting in the absorbance signal from the colored conjugate being greatly amplified to improve the sample detection sensitivity, and to a detection method using the same.

主权项

1. A lateral flow membrane-based immunochromatographic detection sensor, comprising
(a) a conjugate pad containing (i) a first receptor capable of binding specifically to an analyte in a sample; and (ii) a light-absorbing metal particle capable of binding to the first receptor or bound to the first receptor,
wherein the light-absorbing metal particle binds with the first receptor before or during the sample migration, thereby forming a colored conjugate, and the colored conjugate moves together with the sample during the sample migration along the following membrane; (b) a membrane containing
(i) a sample line region having a second receptor capable of binding specifically to the analyte,wherein the second receptor immobilized to the sample line region captures the analyte bound to the first receptor of the colored conjugate, thereby forming a first colored conjugate band; and(ii) a control line region having a third receptor capable of binding specifically to the first receptor,wherein the third receptor immobilized to the control line region captures the first receptor of the colored conjugate which is not bound to the analyte, thereby forming a second colored conjugate band; (c) a substrate provided under the membrane; and (d) two optical waveguides for transmitting the light as a probe beam, while being positioned between the membrane and the substrate, wherein each optical waveguide is entirely overlapped with the sample line region or the control line region, wherein energy from the evanescent wave is generated by the probe beam, at the interface between the membrane and the optical waveguide, and the colored conjugate band absorbs the energy of the evanescent wave, thereby reducing the amount of the energy of the evanescent wave and reducing the intensity of the output probe beam, depending on the amount of the colored conjugates present in the colored conjugate band.