METHOD FOR ENHANCING EXTRACTION EFFICIENCY OF MIRNA FROM CELLS BY THE ADDITION OF TRITON X-100

摘要

The present invention relates to a method for improving detection efficiency of miRNAs existing in cells in trace amounts by adding Triton X-100. In accordance with the present invention, miRNAs existing in a sample in trace amounts can be quantitatively analyzed in short time. Further, the miRNA detection method according to the present invention wherein Triton X-100 is used can improve detection efficiency by about 2 times as compared to when only a TRIzol reagent is used.

主权项

1. A method for improving detection efficiency of miRNAs existing in cells in trace amounts by adding Triton X-100 (polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether), comprising:
lysing cells using a cell lysis buffer containing a TRIzol reagent; adding Triton X-100 and incubating the cells; obtaining a cell extract through centrifugation; and detecting miRNAs from the cell extract.